The ARISE program aims to enable large-scale biomonitoring using, among others, DNA methods. We strive to create an infrastructure that enables high-throughput species identification, as well as molecular biodiversity discovery and monitoring. With plans to sequence 350,000 specimens, we aim to cover at least 95% of all Dutch species, ranging from plants and insects we see around us, to fungi hidden in our soil and the multitude of planktonic organisms inhabiting our water. This target list is where we keep track of which species of the Dutch flora and fauna have been sequenced for the DNA reference library, both in-house at Naturalis Biodiversity Center and the Westerdijk Fungal Biodiversity Institute and at other laboratories around the world. By comparing all that has been sequenced to the current list of Dutch species as recorded by Nederlandsesoorten.nl, we can find the gaps in our reference database, and create a target list for species to be collected and sequenced within the ARISE program.